RELEVANT PUBLICATIONS & PRESENTATIONS.

• A platform for rapid and efficient engineering of multi-edited clonal iPSC lines for allogeneic T-cells therapies

  Laura Prochazka, Justin Yoo, Zahra Sahaf, Dhaarini Murugan, Julia Bershadsky, Dylan Lee, Elisa Martinez, Leanne Jamieson, Lifu Sheng, Dhanya Vijayan, Charlie Liu, April Marple, Ian Cardle, Sommer Apelu, Valarie Val, Katrina Kaur, Rich Carpenedo, Siemon Ng, Liz Csaszar, Siddharth Chandrasekaran, Emily Titus, Chris Bond | ISSCR 2024 Hamburg. July 10 – 13, 2024

• Generation of functional CD8+ T cells from iPSCs in a scalable stirred tank bioreactor via notch signaling provided by DLL4/VCAM-coupled microbeads to support the development of off-the-shelf CAR-T cell therapeutics

  Shri Joshi, Vaisakh Rajan, Chloe Hurling, Richard Carpenedo, Laxsha Thaya, Alessia Pallaoro, Manu Thomas, Hiofan Hoi, Eileen Yoshida, Dan Kirouac, Avisek Deyati, Siddarth Chandrasekaran, Valerie Wall, Libin Abraham, Sommer Apelu, Michael Hunter Cadell, Amanda AuYeung, Omar Subedar1, Muluken S. Belew, Liz Csaszar, Steven Woodside, Emily Titus, Christopher Bond | ISCT 2024 Vancouver. May 29 – June 1, 2024

• Preclinical in vitro and in vivo evaluation of CD8αβ+ CD19 CAR iPSC T cells generated in a TCR signal independent manner using DLL4/VCAM-coupled microbeads

  Muluken S. Belew, Richard Carpenedo, Alessia Pallaoro, Hiofan Hoi, Avisek Deyati, Daniel C. Kirouac, Siddarth Chandrasekaran, Valerie Wall, Libin Abraham, Sommer Apelu, Michael Hunter Cadell, Amanda AuYeung, Omar Subedar, Elisa Martinez, Shri Joshi, Elizabeth Csaszar, Steven Woodside, Emily Titus, Christopher Bond | AACR Annual Meeting 2024. April 5-10, 2024

• Presentation of DLL4 and VCAM on paramagnetic beads replicates thymic Notch signaling and enables differentiation of functional iPSC derived TCR T-cells in vitro

  Siddarth Chandrasekaran, Melanie Kardel, Elisa Martinez, Nabil Zeidan, Avisek Deyati, Valerie Wall, Dylan Lee, Negin Farahani, Amanda Au Yeung, Omar Subedar, Michael Cadell, Sommer Apelu, Laura Prochazka, Zahra Sahaf, Angela Duong, Cole Zmurchok, Boyoung Yoon, Dan Kirouac, Muluken Belew, Jasdeep Mann, Deepika Rajesh, Chris Bond | Society for Immunotherapy of Cancer (SITC) 2023. November 1–5, 2023

• Generation of Functional CD8ab T-cells from iPSCs via Recapitulation of Thymic Notch Signaling Using DLL4/VCAM1 Microbeads

  Elisa Martinez, Rich Carpenedo, Avisek Deyati, Libin Abraham, Marc Ouellette, Elham Afshinmanesh, Laura Prochazka, Boyoung Yoon, Rebecca De Souza, Valerie Wall, Melanie Kardel, Dan Kirouac, Jasdeep Mann, Siddarth Chandrasekaran, Emily Titus, Chris Bond, Deepika Rajesh | ISSCR, Jun 14, 2023

• Scalable Production of Induced Pluripotent Stem Cell-Derived CD8+ Cells in Stirred Tank Reactors Using DLL4/VCAM-1 Coated Beads

  Vaisakh Rajan, Richard Carpenedo, Cole Zmurchok, Chloe Hurling, Laxsha Thaya, Alessia Pallaoro, Manu Thomas, Hiofan Hoi, Eileen Yoshida, Valerie Wall, Sommer Apelu, Michael Hunter Cadell, Sidd Chandrasekaran, Dan Kirouac, Shri Joshi, Liz Csaszar, Steven Woodside, Chris Bond, Emily Titus | ISCT, Jun 1, 2023

• High Yield and Purity Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) to Hematopoietic Progenitor Cells (HPCs) in a Scalable Stirred Tank Bioreactor

  Taylor Perkins, Melanie Kardel, Boyoung Yoon, Charlie Liu, Chloe Hurling, Shri Joshi, Liz Csaszar, Deepika Rajesh, Emily Titus | ISCT, June 1, 2023

• Deconvolution of Clinical Variance in CAR-T Cell Pharmacology and Response

  Daniel C. Kirouac, Cole Zmurchok, Avisek Deyati, Jordan Sicherman, Peter W. Zandstra, Chris Bond | ASGCT, May 18, 2023

• Generation of Functional CD8ab T Cells from iPSCs in a TCR-Signal Independent Manner Via Supra-Physiological Notch Signaling Provided by DLL4/VCAM-Coupled Microbeads

  Richard Carpenedo, Cole Zmurchok, Avisek Deyati, Elham Afshinmanesh, Alessia Pallaoro, Hiofan Hoi, Boyoung Yoon, Mel Kardel, Marc Ouellette, Valerie Wall, Libin Abraham, Siddarth Chandrasekaran, Steven Woodside, Deepika Rajesh, Emily Titus, Chris Bond & Daniel Kirouac | ASGCT, May 17, 2023

• Deconvolution of clinical variance in CAR-T cell pharmacology and response.

  Kirouac DC, Zmurchok C, Deyati A, Sicherman J, Bond C, Zandstra PW. Nat Biotechnol. 2023 February 27; https://doi.org/10.1038/s41587-023-01687-x

• T Cell differentiation from induced pluripotent stem cell-derived blood progenitors using DLL4/VCAM beads

  Richard Carpenedo, Cole Zmurchok, Alessia Pallaoro, Hiofan Hoi, Elham Afshinmanesh, Marc Ouellette, Avisek Deyati, Dan Kirouac, Steven Woodside, Deepika Rajesh, Chris Bond, Emily Titus | Keystone—Emerging Cellular Therapies at the Forefront of Cancer Immunotherapy, January 30, 2023

• MAD7-based gene editing platform for rapid and efficient iPSC engineering

  Jasdeep Mann, Laura Prochazka, Justin Yoo, Nabil Zeidan, Zahra Sahaf, Dhaarini Murugan, Sam Lam, Julia Bershadsky, Avisek Deyati, Chris Bond | Keystone—Emerging Cellular Therapies at the Forefront of Cancer Immunotherapy, January 30, 2023

• Generation of Multipotent Lymphoid-Competent CD34+ Hematopoietic Progenitor Cells from Human Induced Pluripotent Stem Cells

  Melanie Kardel, Elisa Martinez, Boyoung Yoon, Avisek Deyati, Marc Ouellette, Dan Kirouac, Chris Bond, Deepika Rajesh. Notch Therapeutics | Keystone—Emerging Cellular Therapies at the Forefront of Cancer Immunotherapy, January 30, 2023

• The heart of the machine: Construction of T cell identity, made accessible.

  Rothenberg EV. J Immunol. 2022 October 1; 209 (7): 1235–1236.

• DLL4 and VCAM1 enhance the emergence of T cell–competent hematopoietic progenitors from human pluripotent stem cells.

  Michaels YS, Edgar JM, Major MC, Castle EL, Zimmerman C, Yin T, Hagner A, Lau C, Hsu HH, Ibañez-Rios MI, Durland LJ, Knapp DJHF, Zandstra PW. Sci Adv. 2022 August 24; 8:34.

• Synthetic gene circuits for cell state detection and protein tuning in human pluripotent stem cells.

  Prochazka L, Michaels YS, Lau C, Jones RD, Siu M, Yin T, Wu D, Jang E, Vazquez-Cantu M, Gilbert PM, Kaul H, Benenson Y, Zandstra PW. Mol Syst Biol. 2022 18: e10886.

• DL4-µbeads induce T-cell lineage differentiation from stem cells in a stromal cell-free system.

  Trotman-Grant AC, Mohtashami M, De Sousa Casal J, Martinez EC, Lee D, Teichman S, Brauer PM, Han J, Anderson MK, Zúñiga-Pflücker JC. Nat Commun. 2021 August 18; 12, 5023.

• Controlled and scalable T cell differentiation from induced pluripotent stem cell (iPSC)-derived blood progenitor cells using the engineered thymic niche technology.

  Woodside S, Kirouac D, Carpenedo R, Pallaoro A, Hoi H, Deyati A, Kardel M, Shukla S, Zuniga-Pflucker JC, Trotman-Grant A, Mohtashami M, Zandstra P. International Society for Stem Cell Research (ISSCR) 2021 Virtual Meeting. June 21–26, 2021.

• Process evolution in cell and gene therapy from discovery to commercialization.

  Csaszar E, Mills S, Zandstra PW. Can J Chem Eng. 2021 April 20. https://doi.org/10.1002/cjce.24141


• In vitro-generated MART-1-specific CD8 T cells display a broader T-cell receptor repertoire than ex vivo naïve and tumor-infiltrating lymphocytes.

  Benveniste PM, Nakatsugawa M, Nguyen L, Ohashi PS, Hirano N, Zúñiga-Pflücker JC. Immunol Cell Biol. 2019 April; 97(4):427-434.

• Generation and molecular recognition of melanoma-associated antigen-specific human γδ T cells.

  Benveniste PM, Roy S, Nakatsugawa M, Chen ELY, Nguyen L, Millar DG, Ohashi PS, Hirano N, Adams EJ, Zúñiga-Pflücker JC. Sci Immunol. 2018 December 14; 3(30):eaav4036.

• Commercial Scale Manufacturing of Allogeneic Cell Therapy.

  Pigeau GM, Csaszar E, Dulgar-Tulloch A. Front Med (Lausanne). 2018 August 22; 5:233.

• Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence.

  Rahman N, Brauer PM, Ho L, Usenko T, Tewary M, Zúñiga-Pflücker JC, Zandstra PW. Nat Commun. 2017 May 25; 8:15380.

• Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1.

  Shukla S, Langley MA, Singh J, Edgar JM, Mohtashami M, Zúñiga-Pflücker JC, Zandstra PW. Nat Methods. 2017 May; 14(5):531-538.

• Modeling altered T-cell development with induced pluripotent stem cells from patients with RAG1-dependent immune deficiencies.

  Brauer PM, Pessach IM, Clarke E, Rowe JH, Ott de Bruin L, Lee YN, Dominguez-Brauer C, Comeau AM, Awong G, Felgentreff K, Zhang YH, Bredemeyer A, Al-Herz W, Du L, Ververs F, Kennedy M, Giliani S, Keller G, Sleckman BP, Schatz DG, Bushman FD, Notarangelo LD, Zúñiga-Pflücker JC. Blood. 2016 August 11; 128(6):783-93.

• A Multi-Lineage Screen Reveals mTORC1 Inhibition Enhances Human Pluripotent Stem Cell Mesendoderm and Blood Progenitor Production.

  Nazareth EJP, Rahman N, Yin T, Zandstra PW. Stem Cell Reports. 2016 May 10; 6(5):679-691.

• Quality cell therapy manufacturing by design.

  Lipsitz YY, Timmins NE, Zandstra PW. Nat Biotechnol. 2016 April; 34(4):393-400.

• High-throughput fingerprinting of human pluripotent stem cell fate responses and lineage bias.

  Nazareth EJ, Ostblom JE, Lücker PB, Shukla S, Alvarez MM, Oh SK, Yin T, Zandstra PW. Nat Methods. 2013 December; 10(12):1225-31.

• Characterization in vitro and engraftment potential in vivo of human progenitor T cells generated from hematopoietic stem cells.

  Awong G, Herer E, Surh CD, Dick JE, La Motte-Mohs RN, Zúñiga-Pflücker JC. Blood. 2009 July 30; 114(5):972-82.

• The OP9-DL1 system: generation of T-lymphocytes from embryonic or hematopoietic stem cells in vitro.

  Holmes R, Zúñiga-Pflücker JC. Cold Spring Harb Protoc. 2009 February.

• T-cell development made simple.

  Zúñiga-Pflücker JC. Nat Rev Immunol. 2004 January; 4(1):67-72.

• In vitro generation of T lymphocytes from embryonic stem cell-derived prehematopoietic progenitors.

  de Pooter RF, Cho SK, Carlyle JR, Zúñiga-Pflücker JC. Blood. 2003 September 1; 102(5):1649-53.

• Deficient T cell fate specification in mice with an induced inactivation of Notch1.

  Radtke F, Wilson A, Stark G, Bauer M, Meerwijk JV, MacDonald HR, Aguet M. Immunity. 1999 May; 10(5):547-58.